Enzyme Pectate lyase

Shipping temperature: ambient

Storage temperature: <-10°C

Formulation: in 50% (v/v) glycerol

Physical form: solution

EC 4.2.2.2
CAZy Family: PL1
CAS: 9015-75-2

Synonyms: pectate lyase; -α-D-galacturonan lyase

Source: Aspergillus sp.
​Highly purified

Molecular weight: 45,000

Concentration: supplied at ~ 1,400 U/mL

Specific activity:
~ 180 U/mg (40°C, pH 8.0 on polygalacturonic acid)

Unit definition:
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one micromole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8,0 at 40°C

Specificity:
Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

Temperature optima: 55°C
pH optima: 8

Stability: > 4 years at 4°C or - 10°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research

Data Booklet Safety data sheet

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